Tth ssb

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WebDec 25, 2013 · INTRODUCTION. Single-stranded (ss) DNA binding (SSB) proteins are ubiquitous and found in bacterial, archaeal and eukaryotic cells, mitochondria, phages and … WebIncluding ET SSB in TtAgo reactions can improve cleavage of higher GC content substrates In some cases the addition of ET SSB ( NEB #M2401 ) can aid in the cleavage of higher GC content dsDNA substrates.1 ET SSB must be titrated to find an optimal concentration for the application, and is only found to be beneficial when guides are fully complementary to the …

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WebET SSB can be used in applications that require extremely high temperature conditions, such as nucleic acid amplification and sequencing. Figure 1: Improved multiplex PCR by ET … WebTtAgo is a prokaryotic argonaute from the Gram-negative, thermophilic bacterium Thermus thermophilus which functions as a DNA-guided endonuclease when provided with a 16–18 nucleotide long 5′-phosphorylated single-stranded DNA oligonucleotide guide. The RNase H-like active site of TtAgo requires divalent Mg 2+ (or Mn 2+) (See product usage note #1) … chinese windsor ontario

Single-Stranded DNA Binding Protein (SSB) - Thermo Fisher

WebExtreme Thermostable SSB is a single-stranded DNA binding protein isolated from a hyperthermophilic microorganism. It remains fully active after incubation at 95°C for up to 60 minutes. Due to its ability to withstand extremely high temperature environments, ET SSB can be used in applications that require extremely high temperature conditions, such as … WebOct 1, 2002 · Single-stranded DNA-binding proteins (SSBs) play essential roles in DNA replication, recombination, and repair in bacteria, archaea, and eukarya.We report here the identification, expression, and purification of the SSB-like proteins of the thermophilic bacteria Thermus thermophilus and T. aquaticus.The nucleotide (nt) sequence revealed … WebAfter binding single-stranded DNA, SSB destabilizes helical duplexes, thereby allowing DNA polymerases to access their substrate more easily. SSB is involved in DNA replication and … chinese wine bottle holder

Use of Native Gels to Measure Protein Binding to SSB

ssDNA Binding Proteins NEB

WebApr 5, 2008 · The thermophilic bacterium Thermoanaerobacter tengcongensis has two single-stranded DNA-binding (SSB) proteins, designated TteSSB2 and TteSSB3. In a SSB complementation assay in Escherichia coli, only TteSSB3 took over the in vivo function of EcoSSB. We have cloned the ssb genes obtained by PCR and have developed E. coli … WebDrpSSB is the most thermostable SSB-like protein identified to date, offering an attractive alternative for TaqSSB and TthSSB in their applications for molecular biology methods and analytical purposes. We report the identification and characterization of the single-stranded DNA-binding protein (SSB) from the mesophile and highly radiation-resistant Deinococcus … grange co op grants pass hoursWebDescription. Thermus aquaticus derived single-stranded DNA binding protein (SSB) is a thermostable protein which binds to single-stranded DNA with high specificity but does … chinese wine for sale

"WebssDNA Binding Proteins. Product Listing Product Overview. NEB offers highly thermostable ssDNA binding proteins that are ideal for nucleic acid amplification and sequencing (Tth … " - Tth ssb

Tth ssb

Systematic Functional Comparative Analysis of Four Single

WebJan 24, 2013 · The four SSB proteins were divided into two groups based on primary sequence analysis. tth and bl21 were similar to that of E. coli (NCBI LOCUS: 1EYG_D), … WebJan 1, 2005 · Abstract. In this paper we report that the inclusion of heat-resistant RecA protein from a thermophilic bacteria, Thermus thermophilus, and its cofactor (ATP) in PCR effectively eliminates non-specific PCR products.The effect of RecA protein, which catalyzes pairing between homologous DNA molecules with great fidelity in genetic recombination, …

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WebJun 1, 2007 · philus (Tth SSB), and E. coli (Eco SSB). The analysis show s. that Thermus / Deinoc occus SSBs are highly conserved. The. protein sequence for Dmu SSB protein shar es 73% identity. WebSSB, which is also conserved in Thermus or Deinococcus SSBs, is involved in its interaction with several proteins that partici-pate in DNA metabolism (28–31). The activity of SSB-inter-acting proteins is reportedly stimulated by their interactions with ssDNA-bound SSB (32, 33).

WebIncluding ET SSB in TtAgo reactions can improve cleavage of higher GC content substrates In some cases the addition of ET SSB ( NEB #M2401 ) can aid in the cleavage of higher … WebMay 17, 2006 · We have found that the mutant protein of single-stranded DNA binding protein (SSB) from Thermus thermophilus (Tth) HB8 enhances the efficiency of …

WebMay 17, 2006 · The wild-type T.thermophilus SSB and its mutant were prepared as described in Materials and Methods, which were named TthSSB and TthSSB-255 protein, respectively. Each step of the purification of Tth SSB was analyzed by 12.5% SDS–PAGE with CBB staining ( Figure 1 ). WebSSB is known to bind ssDNA, protecting it from cleavage by endonucleases. 4 Furthermore, SSB has been found to reduce secondary structure of ssDNA, improve PCR specificity, …

WebThis “step up” feature of the SSB reflects the objective of the Savings Bond programme, which is to facilitate long-term savings and investment. Coupon rates for each issuance of Savings Bonds are determined such that the average annual compounded return over an investor’s investment period (e.g. 5 years) is linked to the yield of an SGS ...

WebAug 15, 2009 · T. thermophilus SSB (TthSSB)–F255P and wild-type TthSSB proteins were prepared as described previously [8]. The proteins were shown to be 99% pure. The … grange co op in white city oregonWebSingle-Stranded DNA Binding Protein (SSB) 以高亲和力协同结合单链 DNA，其与双链 DNA 的结合度不高。与单链 DNA 结合后，SSB 使螺旋二聚体变得不稳定，因此 DNA 聚合酶可以更容易地接触到底物。SSB 参与 DNA 复制并在体内重组。目前它也已用于在电镜下显示单链 DNA。PCR 可通过将这种结合蛋白添加到反应液中而 ... grange co-op business office medford orWebWe describe a procedure to detect protein binding to SSB by polyacrylamide gel electrophoresis under non-denaturing conditions. As an example, we show the interaction of Thermus thermophilus (Tth) SSB with its cognate RecO protein.The interaction is detected as decay of the band corresponding to SSB by addition of RecO. grange co-op in grants pass orWebJun 1, 2024 · 2024-01-01. In the present paper, we propose a new frequency-shift keying continuous phase modulation (FSK-CPM) scheme having, by essence, the interesting feature of single-sideband ( SSB) spectrum providing a very compact frequency occupation. First, the original principle, inspired from quantum physics (levitons), is presented. chinese windsor nsWebSSB is known to bind ssDNA, protecting it from cleavage by endonucleases. 4 Furthermore, SSB has been found to reduce secondary structure of ssDNA, improve PCR specificity, … grange co-op issaquah waWebJan 1, 2006 · The Tth SSB-255 mutant protein was also purified using the same strategy (data not shown). The final products were shown to be 99% pure. Properties of Tth SSB … grange coop in white city oregonWebExcellgen Tth-SSB [EG-171] - Return to List . Next chinese windsor va